HOMEPRODUCTSSERVICESCOMPANYCONTACTFAQResearchDictionaryPharmaMobileSign Up FREE or Login

In vitro formation of different tubulin polymers from purified tubulin of Ehrlich ascites tumor cells.

Abstract
Preparations of cycled tubulin from Ehrlich ascites tumor cells contain several accessory proteins; once or twice cycled microtubule preparations are usually composed of fibers 10 nm in diameter, but lack vimentin. Highly purified tubulin consists of alpha- and beta-tubulin and a minor component which was identified by peptide mapping as a second beta-chain. This pure tubulin is able to form in vitro at low concentrations (1 mg protein/ml) fibers of about 10 nm width, and at higher concentrations (3.5 mg protein/ml) normal microtubules.
AuthorsK H Doenges, H P Zimmermann, M Flemming, D Schroeter
JournalFEBS letters (FEBS Lett) Vol. 151 Issue 2 Pg. 286-90 (Jan 24 1983) ISSN: 0014-5793 [Print] NETHERLANDS
PMID6832357 (Publication Type: Journal Article)
Chemical References
  • Macromolecular Substances
  • Tubulin
Topics
  • Animals
  • Carcinoma, Ehrlich Tumor (ultrastructure)
  • Macromolecular Substances
  • Mice
  • Microscopy, Electron
  • Microtubules (ultrastructure)
  • Molecular Weight
  • Tubulin (isolation & purification, metabolism)

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research network!


Choose Username:
Email:
Password:
Verify Password: