Abstract |
Preparations of cycled tubulin from Ehrlich ascites tumor cells contain several accessory proteins; once or twice cycled microtubule preparations are usually composed of fibers 10 nm in diameter, but lack vimentin. Highly purified tubulin consists of alpha- and beta-tubulin and a minor component which was identified by peptide mapping as a second beta-chain. This pure tubulin is able to form in vitro at low concentrations (1 mg protein/ml) fibers of about 10 nm width, and at higher concentrations (3.5 mg protein/ml) normal microtubules.
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Authors | K H Doenges, H P Zimmermann, M Flemming, D Schroeter |
Journal | FEBS letters
(FEBS Lett)
Vol. 151
Issue 2
Pg. 286-90
(Jan 24 1983)
ISSN: 0014-5793 [Print] England |
PMID | 6832357
(Publication Type: Journal Article)
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Chemical References |
- Macromolecular Substances
- Tubulin
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Topics |
- Animals
- Carcinoma, Ehrlich Tumor
(ultrastructure)
- Macromolecular Substances
- Mice
- Microscopy, Electron
- Microtubules
(ultrastructure)
- Molecular Weight
- Tubulin
(isolation & purification, metabolism)
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