In vitro formation of different tubulin polymers from purified tubulin of Ehrlich ascites tumor cells.

Preparations of cycled tubulin from Ehrlich ascites tumor cells contain several accessory proteins; once or twice cycled microtubule preparations are usually composed of fibers 10 nm in diameter, but lack vimentin. Highly purified tubulin consists of alpha- and beta-tubulin and a minor component which was identified by peptide mapping as a second beta-chain. This pure tubulin is able to form in vitro at low concentrations (1 mg protein/ml) fibers of about 10 nm width, and at higher concentrations (3.5 mg protein/ml) normal microtubules.
AuthorsK H Doenges, H P Zimmermann, M Flemming, D Schroeter
JournalFEBS letters (FEBS Lett) Vol. 151 Issue 2 Pg. 286-90 (Jan 24 1983) ISSN: 0014-5793 [Print] NETHERLANDS
PMID6832357 (Publication Type: Journal Article)
Chemical References
  • Macromolecular Substances
  • Tubulin
  • Animals
  • Carcinoma, Ehrlich Tumor (ultrastructure)
  • Macromolecular Substances
  • Mice
  • Microscopy, Electron
  • Microtubules (ultrastructure)
  • Molecular Weight
  • Tubulin (isolation & purification, metabolism)

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