Fasciola hepatica: effects of diamfenetide free amine on in vitro physiology, biochemistry, and morphology.

Abstract

Short-term (1-3 hr) incubations in vitro of immature and adult Fasciola hepatica with 10(-4) to 10(-5) M free amine of diamfenetide (DPT-FA) demonstrated a time/dose-dependent, irreversible paralysis that involved an increase in muscular tension and decrease in contraction amplitude. The following events occurred preceding or concomitant with the paralysis: influx of Na+, decrease in surface membrane potential, increase in wet weight, swellings on the ventral surface, and inhibition of 3-O-methyl glucose transport. These events were all consistent with a disturbance in surface membrane functions. The effects of DPT-FA were more severe in immature flukes (3-5 weeks postinfection) than adults which agrees with observed in vivo efficacy.

• Authors: R S Rew, R H Fetterer, T C Martin
• Journal: Experimental parasitology (Exp Parasitol) Vol. 55 Issue 2 Pg. 159-67 (Apr 1983) ISSN: 0014-4894 [Print] United States
• PMID: 6832277 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Acetanilides
• Methylglucosides
• 3-O-Methylglucose
• Serotonin
• Sodium
• Potassium
• Diamfenetide

Topics

• 3-O-Methylglucose
• Acetanilides (pharmacology)
• Animals
• Biological Transport (drug effects)
• Diamfenetide (pharmacology)
• Dose-Response Relationship, Drug
• Fasciola hepatica (drug effects, physiology, ultrastructure)
• Membrane Potentials (drug effects)
• Methylglucosides (metabolism)
• Muscle Contraction (drug effects)
• Potassium (metabolism)
• Serotonin (pharmacology)
• Sodium (metabolism)
• Time Factors