Kinetic parameters of aspartate transcarbamylase in human normal and tumoral cell lines.

Abstract

The kinetic parameters of aspartate transcarbamylase activity were determined in dialyzed extracts coming from ten different human normal and tumoral cell lines (three fibroblasts, four melanomas, and three colorectal carcinomas). Specific activities do not correlate with the malignant character of the cells but rather with the fact that the cells divide actively or not. Growth curves show large variations in the cell sensitivity to N-(phosphonacetyl)-L-aspartate (PALA). However, no differences in substrate affinity or PALA sensitivity of the aspartate transcarbamylase activities present in the corresponding cell extracts could be detected. Thus, the different human cell susceptibilities to PALA do not result from an intrinsic property of aspartate transcarbamylase. Cell death under the influence of PALA does not correlate with the tumoral or normal character of the cells.

• Authors: J Baillon, M Guichard, E P Malaise, G Hervé
• Journal: Cancer research (Cancer Res) Vol. 43 Issue 5 Pg. 2277-82 (May 1983) ISSN: 0008-5472 [Print] United States
• PMID: 6831451 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Cell Extracts
• Organophosphorus Compounds
• Aspartic Acid
• sparfosic acid
• Aspartate Carbamoyltransferase
• Phosphonoacetic Acid

Topics

• Aspartate Carbamoyltransferase (antagonists & inhibitors)
• Aspartic Acid (analogs & derivatives, pharmacology)
• Cell Count
• Cell Extracts
• Cell Line
• Cell-Free System (drug effects)
• Humans
• Kinetics
• Neoplasms (enzymology, pathology)
• Organophosphorus Compounds (pharmacology)
• Phosphonoacetic Acid (analogs & derivatives, pharmacology)
• Time Factors