Colon-specific antigen-p (CSAp). II. Further characterization in colorectal and pancreatic cancer.

Abstract

The physicochemical and immunological characteristics of colon-specific antigen-p (CSAp) in plasma and in colorectal and pancreatic tumors were investigated. CSAp in the plasma of a rectal cancer patient and in a colonic carcinoma xenografted in hamsters (GW-39 tumor) appeared to have similar chromatographic properties, being of a molecular size of 4 million or more. The activities of CSAp in both plasma and tumor were similarly destroyed by treatment with a thiol reagent. Finally, identical immunological reactions in radioimmunoassay and gel diffusion tests were obtained between the CSAp's in patient plasma and in GW-39 tumor tissue. Also CSAp in human pancreatic cancers xenografted in nude mice showed a precipitin line of complete identity with CSAp extracted from GW-39 human colonic carcinoma transplants. Thus, the CSAp's found in colorectal cancer patient plasma, in colonic carcinoma, and in pancreatic cancer tissues appear to be immunologically identical.

• Authors: D Shochat, K D Pant, D M Goldenberg
• Journal: Cancer (Cancer) Vol. 50 Issue 5 Pg. 927-31 (Sep 01 1982) ISSN: 0008-543X [Print] United States
• PMID: 6807529 (Publication Type: Comparative Study, Journal Article, Research Support, U.S. Gov't, P.H.S.)

Chemical References

• Antigens, Neoplasm
• colon-specific antigen

Topics

• Animals
• Antigens, Neoplasm (analysis)
• Cell Line
• Chromatography, Gel
• Colonic Neoplasms (immunology)
• Cricetinae
• Humans
• Immunodiffusion
• Mice
• Mice, Nude
• Neoplasm Transplantation
• Neoplasms, Experimental (immunology)
• Pancreatic Neoplasms (immunology)
• Radioimmunoassay
• Rectal Neoplasms (immunology)