Primary and secondary structure of U2 snRNA.

Abstract

With the improved rapid sequencing techniques, the earlier sequence of U2 RNA of Novikoff hepatoma (Shibata et al, J. Biol. Chem. 250, 3909-3920, 1975) was reanalyzed and modified. The improved sequence of U2 RNA is 188 (or 189) nucleotides long and is in register with a characterized U2 RNA pseudogene (Denison et al, PNAS 78, 810-814, 1981) except for an 11 nucleotide sequence (nucleotides 147-157) which is absent from the pseudogene. From these results, a secondary structure of U2 RNA is proposed which is supported by the preferred cleavage sites with T1-RNase, RNase A and S1 nuclease. Isolated U2 RNA was cleaved by T1-RNase preferentially at positions 64 and 164, whereas U2 RNA in U2-snRNP was cleaved only at position 64, indicating that position 164 is protected in U2-snRNP. As with U1 RNA (Epstein et al, PNAS 78, 1562-1566, 1981) the 5'-end of isolated U2 RNA was not preferentially cleaved by T1-RNase.

• Authors: R Reddy, D Henning, P Epstein, H Busch
• Journal: Nucleic acids research (Nucleic Acids Res) Vol. 9 Issue 21 Pg. 5645-58 (Nov 11 1981) ISSN: 0305-1048 [Print] England
• PMID: 6796940 (Publication Type: Journal Article, Research Support, U.S. Gov't, P.H.S.)

Chemical References

• Oligoribonucleotides
• RNA, Neoplasm
• Ribonucleoproteins
• Ribonucleases
• Ribonuclease T1

Topics

• Animals
• Base Sequence
• Liver Neoplasms, Experimental (analysis)
• Nucleic Acid Conformation
• Oligoribonucleotides (analysis)
• RNA, Neoplasm
• Rats
• Ribonuclease T1
• Ribonucleases
• Ribonucleoproteins