Aminoguanidine reversal of the inhibitory effects of ornithine analogs on the in vitro clonogenic survival of the R3327AT prostate-derived tumor.

Abstract

In in vitro studies with the Copenhagen rat prostate-derived tumor cell lines, the activity of ornithine decarboxylase (ODC) of these lines were as sensitive to inhibition by alpha-difluoromethylornithine (alpha-DFMO) as normal prostatic tissue. The in vitro growth of the tumor in calf serum was inhibited by ODC inhibitors alpha-methylornithine and alpha-difluoromethylornithine. This inhibition was partially reversed by the diamine oxidase inhibitor aminoguanidine. Further, alpha-DFMO was not inhibitory to the growth of these cells in sera which lacked diamine oxidase activity, but was inhibitory when diamine oxidase was added.

• Authors: W D Heston, D W Lazan, W R Fair
• Journal: Cancer letters (Cancer Lett) Vol. 11 Issue 4 Pg. 323-30 (Feb 1981) ISSN: 0304-3835 [Print] Ireland
• PMID: 6794904 (Publication Type: Journal Article, Research Support, U.S. Gov't, P.H.S.)

Chemical References

• Guanidines
• Ornithine Decarboxylase Inhibitors
• Ornithine
• Amine Oxidase (Copper-Containing)
• Carboxy-Lyases
• Ornithine Decarboxylase
• pimagedine
• Putrescine
• Eflornithine

Topics

• Amine Oxidase (Copper-Containing) (antagonists & inhibitors, physiology)
• Animals
• Carboxy-Lyases (antagonists & inhibitors)
• Cell Line
• Eflornithine
• Guanidines (pharmacology)
• Male
• Ornithine (analogs & derivatives, pharmacology)
• Ornithine Decarboxylase (analysis)
• Ornithine Decarboxylase Inhibitors
• Prostatic Neoplasms (drug therapy, enzymology)
• Putrescine (pharmacology)
• Rats