Characterization of the viral RNA species of prototype dengue viruses.

Abstract

Dengue virus isolates have been grown in the Igarashi clone C6/36 Aedes albopictus cells in amounts suitable for biochemical studies. Oligonucleotide fingerprint analyses have been used to characterize the virion 40 S RNA species of the four prototype dengue (DEN) serotype viruses: DEN 1, Hawaiian strain; DEN 2, New Guinea C strain (both insect and mouse brain passaged derivatives); DEN 3, H-87 strain; and DEN 4, H-241 strain. Comparisons of the largest oligonucleotides derived by ribonuclease T1 digestion of these 40 S DEN virion RNA species indicate that ther are few, if any, large oligonucleotides that are homologous between any two fo the four dengue prototype strains. Preliminary analyses of the 40 S RNA 5' terminal sequence of DEN 3 virion RNA indicate that it has the composition of m7GpppAmpXp.

• Authors: A C Vezza, L Rosen, P Repik, J Dalrymple, D H Bishop
• Journal: The American journal of tropical medicine and hygiene (Am J Trop Med Hyg) Vol. 29 Issue 4 Pg. 643-52 (Jul 1980) ISSN: 0002-9637 [Print] United States
• PMID: 6773429 (Publication Type: Journal Article, Research Support, U.S. Gov't, Non-P.H.S., Research Support, U.S. Gov't, P.H.S.)

Chemical References

• Oligonucleotides
• RNA, Viral

Topics

• Aedes
• Animals
• Dengue Virus (analysis)
• Haplorhini
• Humans
• Macaca mulatta
• Oligonucleotides (analysis)
• RNA, Viral (analysis)
• Species Specificity