Time dependence of the in vitro cytotoxicity of hexamethylmelamine and its metabolites.

Abstract

The cytotoxicity of hexamethylmelamine (HMM) and its metabolites pentamethylmelamine (PMM), N,2,2,4,6-tetramethylmelamine (TMM) and hydroxymethylpentamethylmelamine (HMPMM) and of the alkylating agent triethylenemelamine (TEM) were studied on a cell line derived from a human ovarian cancer, by measuring [3H]TdR uptake. After 24 h of incubation all the tested compounds inhibited [3H]TdR uptake, but only at a concentration of 100 micrograms/ml. However, after 120 h incubation, concentrations of 0.1--10 micrograms/ml resulted in highly significant cytotoxicity. HMPMM and TEM were the most active and their effect was not reversed 72 h after their removal. In our in vitro system no metabolism of HMM was observed.

• Authors: M D'Incalci, E Erba, G Balconi, L Morasca, S Garattini
• Journal: British journal of cancer (Br J Cancer) Vol. 41 Issue 4 Pg. 630-5 (Apr 1980) ISSN: 0007-0920 [Print] England
• PMID: 6770884 (Publication Type: Journal Article)

Chemical References

• Triazines
• Altretamine
• Thymidine

Topics

• Altretamine (metabolism, pharmacology)
• Cell Line
• Cells (drug effects, metabolism)
• Female
• Humans
• Ovarian Neoplasms (pathology)
• Thymidine (metabolism)
• Time Factors
• Triazines (pharmacology)