Genes for three Drosophila heat-shock-induced proteins at a single locus.

Abstract

Two plasmids containing cDNA inserts complementary to the messenger RNAs for the 26,000 and 23,000 molecular weight heat-shock-induced proteins from Drosophila melanogaster were identified. Both of these probes hybridized to a site of a major heat-shock-induced puff, 67B. Hybridization by the gel transfer technique with genome DNA that had been cleaved by a restriction endonuclease indicated that the two genes are closely linked. This expectation was validated through isolation of a DNA fragment generated by BamHI endonuclease containing genes for the 26,000 and 23,000 molecular weight heat-shock-induced proteins and also a gene encoding the 27,000 molecular weight heat-shock-induced protein.

• Authors: S C Wadsworth, E A Craig, B J McCarthy
• Journal: Proceedings of the National Academy of Sciences of the United States of America (Proc Natl Acad Sci U S A) Vol. 77 Issue 4 Pg. 2134-7 (Apr 1980) ISSN: 0027-8424 [Print] United States
• PMID: 6769119 (Publication Type: Journal Article, Research Support, U.S. Gov't, P.H.S.)

Chemical References

• DNA, Recombinant
• Proteins

Topics

• Animals
• Chromosome Mapping
• Chromosomes (ultrastructure)
• DNA, Recombinant
• Drosophila melanogaster (genetics)
• Genes
• Genetic Linkage
• Hot Temperature
• Molecular Weight
• Nucleic Acid Hybridization
• Plasmids
• Proteins (genetics)