Counterimmunoelectrophoresis, was performed for serologic testing for
coccidioidomycosis, employing
coccidioidin and
spherulin as
antigens. Results obtained by counterimmunoelectrophoresis were compared with results obtained by
complement-fixation and radial immunodiffusion. Of the 37 sera positive by
complement-fixation, 28 were positive by counter-immunoelectrophoresis utilizing
coccidioidin, whereas only 11 were positive by counterimmunoelectrophoresis utilizing
spherulin (although an additional 14 were positive with counterimmunoelectrophoresis employing
spherulin when the quantity of
spherulin was increased by 50 to 100%). Thirty-three of these
complement-fixation-positive sera were positive by immunodiffusion. Counterimmunoelectrophoresis performed with
coccidioidin detected
antibodies to C. immitis in all patients who had
complement-fixation titers greater than 1:4. When combined with
latex agglutination, counterimmunoelectrophoresis utilizing coccidiodin had an 86% correlation with
complement-fixation for the detection of positive sera.