A clinical assessment of fluorescence polarisation changes in lymphocytes stimulated by phytohaemagglutinin (PHA) in malignant and benign diseases.

Abstract

The modified double-zone SCM technique, developed in these laboratories as an in vitro test for cancer, is based on the differential response to PHA of lymphocytes harvested from two regions of a Ficoll-Triosil gradient. Lymphocyte responses are measured by changes in intracellular fluorescein fluorescence polarisation. We report its continued clinical evaluation in patients hospitalised with malignant and non-malignant diseases, including a blind trial of 78 patients with disorders of the gastrointestinal tract, breast and lung. Overall "false' negative and "false' positive rates from 336 blood samples were 1.8 and 3.3% respectively.

• Authors: J A Pritchard, W H Sutherland, J E Siddall, A J Bater, I J Kerby, T J Deeley, G Griffith, R Sinclair, B H Davies, A Rimmer, D J Webster
• Journal: European journal of cancer & clinical oncology (Eur J Cancer Clin Oncol) Vol. 18 Issue 7 Pg. 651-9 (Jul 1982) ISSN: 0277-5379 [Print] England
• PMID: 6751831 (Publication Type: Clinical Trial, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Phytohemagglutinins

Topics

• Adult
• Aged
• Breast Neoplasms (diagnosis)
• Centrifugation, Density Gradient
• Clinical Trials as Topic
• Diagnosis, Differential
• Female
• Fluorescence Polarization
• Gastrointestinal Diseases (diagnosis)
• Gastrointestinal Neoplasms (diagnosis)
• Humans
• Lung Neoplasms (diagnosis)
• Lymphocyte Activation
• Male
• Middle Aged
• Phytohemagglutinins (pharmacology)
• Respiratory Tract Diseases (diagnosis)