Determination of aminoglutethimide and N-acetylaminoglutethimide in human plasma by reversed-phase liquid chromatography.

Abstract

A liquid chromatography method for the determination of aminoglutethimide and N-acetylaminoglutethimide in human plasma is described. The assay involved precipitation of the plasma proteins using a mixture of acetonitrile and perchloric acid, without an extraction procedure. The supernatant was subjected to chromatography on a 3-micrometers ODS Hypersil column eluted isocratically with 11% acetonitrile in 100 mM ammonium formate buffer, pH 3.5. The absorbance was routinely recorded at 242 nm. The standard curves were linear in the range of 0.1-100 micrograms/ml, and the lower detection limit was approximately 0.1 microgram/ml for aminoglutethimide and its plasma metabolite N-acetylaminoglutethimide. The precision of the method, given as the coefficient of variation, was 3.9%. With this method, it was determined that aminoglutethimide and N-acetylaminoglutethimide were present in the plasma of patients receiving single-dose or continuous treatment with aminoglutethimide for breast cancer. No N-formylaminoglutethimide or nitroglutethimide could be demonstrated in the plasma from these patients. Interference from several drugs commonly given to patients with breast cancer was ruled out.

• Authors: J S Schanche, P E Lønning, P M Ueland, S Kvinnsland
• Journal: Therapeutic drug monitoring (Ther Drug Monit) Vol. 6 Issue 2 Pg. 221-6 ( 1984) ISSN: 0163-4356 [Print] United States
• PMID: 6740741 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Aminoglutethimide
• N-acetylaminoglutethimide

Topics

• Aminoglutethimide (analogs & derivatives, blood, therapeutic use)
• Breast Neoplasms (blood, drug therapy)
• Chromatography, High Pressure Liquid (methods)
• Drug Interactions
• Humans