Abstract |
Proliferation of in vitro grown Ehrlich ascites tumor cells is completely inhibited by 0.2-0.4 mM methylglyoxal and 1-2 mM glucosone or galactosone without severely affecting viability ( dye exclusion test); no phase-specific arrest of cell growth is observed. Incorporation of [14C] thymidine into the acid-insoluble fraction of the cells decreases within a few minutes to less than 50% of that in controls in the presence of 0.4 mM methylglyoxal, and 2 mM glucosone or galactosone causes a comparable inhibition of DNA synthesis after 2 h or 4 h, respectively. The action of 0.4 mM methylglyoxal inhibits incorporation of [14C] leucine within a few minutes by more than 70%, while 2 mM glucosone and galactosone are significantly less effective (50%-60% inhibition after 12 h). While methylglyoxal and galactosone do not severely affect lactate production of the cells, 2 mM glucosone reduces glycolysis by 60%-70%; ATP/ ADP ratios did not fall below 3.5 in the presence of the inhibitors (controls 4-6). It is suggested that the reaction potentialities of the oxaldehyde function of the inhibitors play an important role in their growth-inhibitory activity, besides exerting a specific effect on hexokinase ( glucosone) and UTP-trapping activity.
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Authors | K A Reiffen, F Schneider |
Journal | Journal of cancer research and clinical oncology
(J Cancer Res Clin Oncol)
Vol. 107
Issue 3
Pg. 206-10
( 1984)
ISSN: 0171-5216 [Print] Germany |
PMID | 6736108
(Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Aldehydes
- DNA, Neoplasm
- Ketoses
- Neoplasm Proteins
- glucosone
- galactosone
- Adenosine Diphosphate
- Pyruvaldehyde
- Adenosine Triphosphate
- Galactose
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Topics |
- Adenosine Diphosphate
(metabolism)
- Adenosine Triphosphate
(metabolism)
- Aldehydes
(pharmacology)
- Animals
- Carcinoma, Ehrlich Tumor
(metabolism, pathology)
- Cell Division
(drug effects)
- Cell Survival
(drug effects)
- DNA, Neoplasm
(biosynthesis)
- Energy Metabolism
(drug effects)
- Female
- Galactose
(analogs & derivatives, pharmacology)
- Ketoses
(pharmacology)
- Mice
- Mice, Inbred Strains
- Neoplasm Proteins
(biosynthesis)
- Pyruvaldehyde
(pharmacology)
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