Short-term regulation of muscarinic acetylcholine receptor binding in cultured nerve cells.

Abstract

Short-term preincubation of mouse neuroblastoma cells (clone N1E-115) with the muscarinic agonist carbamylcholine at 37 degrees C resulted in a significant decrease in the binding of the muscarinic antagonist [3H]N-methylscopolamine. This agonist-induced response reached a steady state at 30 min. It was also dose dependent, saturable and sensitive to temperature, suggesting a receptor-mediated phenomenon. The significance of these findings is discussed.

• Authors: P Feigenbaum, E E El-Fakahany
• Journal: Research communications in chemical pathology and pharmacology (Res Commun Chem Pathol Pharmacol) Vol. 43 Issue 3 Pg. 519-22 (Mar 1984) ISSN: 0034-5164 [Print] United States
• PMID: 6718813 (Publication Type: Journal Article, Research Support, U.S. Gov't, P.H.S.)

Chemical References

• Receptors, Muscarinic
• Scopolamine Derivatives
• Carbachol
• N-Methylscopolamine

Topics

• Animals
• Carbachol (metabolism)
• Clone Cells
• Mice
• N-Methylscopolamine
• Neuroblastoma (metabolism)
• Neurons (metabolism)
• Receptors, Muscarinic (metabolism)
• Scopolamine Derivatives