Sulfated N-(carboxymethyl)chitosans: novel blood anticoagulants.

Abstract

N-(Carboxymethyl)chitosan was subjected to sulfation in a mixture of concentrated sulfuric acid (oleum) and N,N-dimethylformamide, under anhydrous conditions. The resulting product contained 11% of sulfur and degree of substitution: N-acetyl, 42%; N-carboxymethyl, 58%; and sulfate, 100%. Sonication of the sulfated N-(carboxymethyl)chitosan gave two main fractions whose molecular weights were 39,000 and 80,000. In human blood, complexes of sulfated N-(carboxymethyl)chitosan and antithrombin inhibited both thrombin and factor Xa, and produced neither hemolysis nor alterations in erythrocytes and lymphocytes. Sulfated N-(carboxymethyl)chitosan is therefore proposed as a blood anticoagulant.

• Authors: R A Muzzarelli, F Tanfani, M Emanuelli, D P Pace, E Chiurazzi, M Piani
• Journal: Carbohydrate research (Carbohydr Res) Vol. 126 Issue 2 Pg. 225-31 (Mar 15 1984) ISSN: 0008-6215 [Print] Netherlands
• PMID: 6713433 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Anticoagulants
• Indicators and Reagents
• Chitin
• Factor X
• Thrombin
• Factor Xa

Topics

• Anticoagulants (chemical synthesis)
• Blood Coagulation (drug effects)
• Chitin (analogs & derivatives, chemical synthesis, pharmacology)
• Factor X (antagonists & inhibitors)
• Factor Xa
• Humans
• Indicators and Reagents
• Thrombin (antagonists & inhibitors)