Direct evidence of incorporation of 12-O-[20-2H1]tetradecanoylphorbol-13-acetate into artificial membranes as determined by deuterium magnetic resonance.

Abstract

In order to elucidate the manner of interaction of 12-O-tetradecanoylphorbol-13-acetate (TPA), 2H-NMR spectra of [20-2H1]TPA and 31P-NMR spectra were recorded in the presence of multibilayers of dimyristoylphosphatidylcholine (DMPC). Observation of several pairs of quadrupole splittings directly proves the TPA molecules are intercalated into the multibilayers. Further, reduction of the 31P chemical shift anisotropy of DMPC multibilayers by TPA is more pronounced than that of phorbol 12,13-diacetate (PDA) whose activity of tumor promotion is very weak.

• Authors: H Saitô, R Tabeta, M Kodama, C Nagata, Y Sato
• Journal: Cancer letters (Cancer Lett) Vol. 22 Issue 1 Pg. 65-9 (Feb 1984) ISSN: 0304-3835 [Print] Ireland
• PMID: 6697322 (Publication Type: Comparative Study, Journal Article)

Chemical References

• Lipid Bilayers
• Membranes, Artificial
• Phorbol Esters
• Phorbols
• phorbol-12,13-diacetate
• Deuterium
• Tetradecanoylphorbol Acetate
• Dimyristoylphosphatidylcholine

Topics

• Cell Membrane (metabolism)
• Deuterium
• Dimyristoylphosphatidylcholine (chemical synthesis)
• Lipid Bilayers (chemical synthesis)
• Magnetic Resonance Spectroscopy
• Membranes, Artificial
• Phorbol Esters (analysis)
• Phorbols (analysis)
• Tetradecanoylphorbol Acetate (analysis)