Abstract |
An unidentified ninhydrin-positive substance of acidic nature was found in the serum of uremic patients. This substance was isolated from hemodialysate by the methods of ion-exchange chromatography, gel-filtration and paper electrophoresis, and identified as beta-aspartylglycine by amino acid analysis, N-terminal amino acid determination and comparison with authentic sample synthesized in this laboratory. The quantitative determination of beta-aspartylglycine in serum revealed that the serum concentrations of beta-aspartyl- glycine in uremic patients increased much higher than those in normal subjects. The toxicity of beta-aspartylglycine in mice with acute renal failure induced by uranyl acetate was investigated. The mice given more than 1,0 g/kg body weight of beta-aspartylglycine showed behavioral alterations: low response to the stimuli and low activity, and some mice died by the injection of 4.0 g/kg body weight of the peptide. These results suggested that beta-aspartyl- glycine might be a possible factor which influences the development of uremic toxaemia.
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Authors | F Gejyo, Y Kinoshita, G Ito, T Ikenaka |
Journal | Contributions to nephrology
(Contrib Nephrol)
Vol. 9
Pg. 69-77
( 1978)
ISSN: 0302-5144 [Print] Switzerland |
PMID | 668390
(Publication Type: Journal Article)
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Chemical References |
- Dipeptides
- Aspartic Acid
- Glycine
|
Topics |
- Acute Kidney Injury
(chemically induced)
- Animals
- Aspartic Acid
(blood)
- Dipeptides
(blood, isolation & purification, toxicity)
- Disease Models, Animal
- Female
- Glycine
(blood)
- Humans
- Mice
- Uremia
(blood)
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