Lipoamide dehydrogenase in cultured human skin fibroblasts.

Abstract

Lipoamide dehydrogenase was identified in cultured skin fibroblasts of normal individuals and patients with Friedreich's ataxia. The optimum conditions for its assay were defined. Data disclosed a normal range of 36--122 mumol/min/mg protein in control fibroblasts and 61--112 mumol/min/mg protein in patients fibroblasts. Numerous precautions should be taken in handling fibroblast cultures for lipoamide dehydrogenase determination.

• Authors: S B Melançon, L Dallaire, M Potier
• Journal: Clinica chimica acta; international journal of clinical chemistry (Clin Chim Acta) Vol. 87 Issue 1 Pg. 29-34 (Jul 01 1978) ISSN: 0009-8981 [Print] Netherlands
• PMID: 668142 (Publication Type: Journal Article)

Chemical References

• Buffers
• Coenzymes
• Proteins
• Dihydrolipoamide Dehydrogenase

Topics

• Buffers
• Cells, Cultured
• Centrifugation
• Coenzymes (metabolism)
• Dihydrolipoamide Dehydrogenase (metabolism)
• Fibroblasts (enzymology)
• Friedreich Ataxia (enzymology)
• Hot Temperature
• Humans
• Osmolar Concentration
• Proteins (metabolism)
• Reference Values
• Skin (enzymology)
• Time Factors