Effect of cycloleucine on amino acid accumulation by human diploid fibroblasts.

Abstract

Cycloleucine is a synthetic amino acid which produces, in vivo, biochemical abnoramlities comparable to those seen in human cystinuria-lysinuria. The effect of cycloleucine on intracellular accumulation of amino acids overlapping separate transport systems was studied using human diploid fibroblasts subcultures on glass coverslips. The data indicated that alpha-alanine, serine and proline accumulation was inhibited significantly by cycloleucine. The percentage of inhibition was approximately the same. Lysine was less affected by cycloleucine, but this amino acid accumulation proceeded at a rate slower than for neutral amino acids. In vitro, this inhibitory effect seems to be a generalized phenomenon affecting substrates. These results confirm in human fibroblasts data reported for human and rat kidney slices.

• Authors: N Moatti, M Feneant, F Lemonnier, M Gautier, A Lemonnier
• Journal: Clinica chimica acta; international journal of clinical chemistry (Clin Chim Acta) Vol. 86 Issue 3 Pg. 245-9 (Jun 15 1978) ISSN: 0009-8981 [Print] Netherlands
• PMID: 668116 (Publication Type: Journal Article)

Chemical References

• Amino Acids
• Proteins
• Cycloleucine

Topics

• Amino Acids (metabolism, pharmacology)
• Cells, Cultured
• Cycloleucine (pharmacology)
• Fibroblasts (drug effects, metabolism)
• Humans
• Proteins (metabolism)