Relationship between adenylate cytokinin production and Ti plasmid of Agrobacterium tumefaciens.

Abstract

To know whether the tumor-inducing plasmid of Agrobacterium tumefaciens carries genetic information of the biosynthesis of cytokinins, the levels of 6-(3-methyl-2-butenyl-amino)purine (iPAde) and its 4-hydroxy derivative trans-zeatin (trans-Z) and its p-beta-D-ribofuranoside (trans-ZR) produced in media by wild-type virulent strain, plasmid-cured avirulent strain and the deletion mutant were compared. The highest levels of iPAde and trans-Z were found in the culture filtrate of late-log phase growth of plasmid-containing virulent strain, then the levels of iPAde and trans-Z were reduced rapidly at stationary phase. The plasmid-cured avirulent strain and deletion mutant had low levels of iPAde and trans-Z throughout the growth. Results obtained here showed Ti plasmid plays an important role in cytokinin biosynthesis.

• Authors: S Sonoki, Y Ohno, N Kijima, T Hishiyama, H Saito, T Sugiyama, T Hashizume
• Journal: Nucleic acids symposium series (Nucleic Acids Symp Ser) Issue 12 Pg. 111-4 ( 1983) ISSN: 0261-3166 [Print] England
• PMID: 6664842 (Publication Type: Journal Article)

Chemical References

• Cytokinins
• Plant Growth Regulators
• N(6)-(delta(2)-isopentenyl)adenine
• Isopentenyladenosine
• Zeatin
• zeatin riboside
• Adenine
• Adenosine

Topics

• Adenine (analogs & derivatives, biosynthesis)
• Adenosine (analogs & derivatives, biosynthesis)
• Cell Transformation, Neoplastic (pathology)
• Cytokinins (biosynthesis)
• Isopentenyladenosine (analogs & derivatives, biosynthesis)
• Plant Growth Regulators (biosynthesis)
• Plasmids
• Rhizobium (growth & development, metabolism)
• Zeatin (biosynthesis)