Three strains of anaerobic,
dextranase-producing, gram-positive, rod-shaped bacteria were isolated from human
dental plaque associated with root
carious lesions. The isolates produced a molar ratio of
acetate to
lactate from
glucose fermentation ranging from 1.1 to 1.9. Each strain also produced
fructose-6-phosphate phosphoketolase. The isolates were identified as belonging to the genus Bifidobacterium, but from their
carbohydrate fermentation patterns they did not appear to be strains of Bifidobacterium dentium. These microorganisms fermented high-molecular-weight
dextrans. A partial characterization of the
dextranase activity was included in this study and revealed an extracellular
dextranase with a pH optimum of 7.1. Analysis of the
dextran degradation products demonstrated the liberation of saccharides larger than 1
glucose unit. It was concluded that this
enzyme used an endohydrolytic mode of
dextran cleavage.