High-dose pyridoxal supplemented culture medium inhibits the growth of a human malignant melanoma cell line.

The in vitro growth characteristics of a human malignant melanoma cell line cultured in 0.5 mM pyridoxal supplemented medium were studied. Experimentation revealed that the high-dose pyridoxal supplemented medium severely inhibited the growth of melanoma cells over a 72-hour growth period. Additional experimentation showed that cells cultured for 6 hours in the pyridoxal supplemented medium took up 25% less and incorporated 20% less [3H]uridine than control cultures. [3H]Glucose uptake was reduced by 23% at this time point. [3H]Thymidine uptake was inhibited by 12%, but no inhibition was detected in [3H]thymidine incorporation. When the vitamin B6 antagonist 4-deoxypyridoxine (which competes with pyridoxal for pyridoxal kinase) was added to the pyridoxal supplemented medium, the inhibition in [3H]uridine incorporation was reduced from 19% to 6%. However, 4-deoxypyridoxine did not reverse the inhibition of [3H]uridine uptake. These results indicate that pyridoxal and its metabolite, pyridoxal 5'-phosphate, may be involved in the growth regulation of a human malignant melanoma cell line.
AuthorsD M DiSorbo, L Nathanson
JournalNutrition and cancer (Nutr Cancer) Vol. 5 Issue 1 Pg. 10-5 ( 1983) ISSN: 0163-5581 [Print] UNITED STATES
PMID6634429 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Pyridoxal
  • Leucine
  • Glucose
  • Thymidine
  • Uridine
  • Cell Division (drug effects)
  • Cells, Cultured
  • Glucose (metabolism)
  • Humans
  • In Vitro Techniques
  • Leucine (metabolism)
  • Male
  • Melanoma (pathology)
  • Pyridoxal (pharmacology)
  • Thymidine (metabolism)
  • Uridine (metabolism)

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