Glycoprotein biosynthesis during the acute-phase response to inflammation.

Abstract

Inflammation results in an increase in the levels of a variety of glycoproteins in serum. The glycoproteins that respond in this way are usually referred to as acute-phase reactants. Studies on the acute-phase response of rat alpha 1-acid glycoprotein showed that there was an increase in the liver levels of this glycoprotein at 12 h after turpentine inflammation. This was followed by increased serum levels at 48-72 h after inflammation, suggesting a precursor-product relationship between liver and serum alpha 1-acid glycoprotein. Incorporation studies coupled with measurements of synthesis rates of alpha 1-acid glycoprotein showed that increased synthesis was responsible for the acute-phase response of this protein to inflammation. These studies also showed that albumin was a negative acute-phase reactant. The acute-phase response of alpha 1-acid glycoprotein was accompanied by increased liver pools of UDP-N-acetylglucosamine (UDP-GlcNAc) and UDP-N-acetylgalactosamine (UDP-GalNAc) and increased liver activities of glucosamine-6-phosphate synthase and UDP-GlcNAc 2-epimerase. Activities of galactosyl and sialyl transferases in liver were also elevated and serum sialyl transferase was increased substantially in inflammation, suggesting that it may also be an acute-phase reactant. Liver activities of beta-N-acetylhexosaminidase and beta-galactosidase declined by about 50% at 24 h after inflammation; there was evidence that serum levels of these enzymes increased at 24-72 h after inflammation, suggesting that the lysosomal glycosidases may be released from liver during inflammation.(ABSTRACT TRUNCATED AT 250 WORDS)

• Authors: J C Jamieson, H A Kaplan, B M Woloski, M Hellman, K Ham
• Journal: Canadian journal of biochemistry and cell biology = Revue canadienne de biochimie et biologie cellulaire (Can J Biochem Cell Biol) Vol. 61 Issue 9 Pg. 1041-8 (Sep 1983) ISSN: 0714-7511 [Print] Canada
• PMID: 6627106 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Glycoproteins
• Hormones
• Interleukin-1
• Oligosaccharides
• Orosomucoid
• Proteins
• Serum Albumin
• leukocyte endogenous mediator
• Sialyltransferases

Topics

• Animals
• Glycoproteins (biosynthesis)
• Golgi Apparatus (enzymology)
• Hormones (blood)
• Humans
• Inflammation (metabolism)
• Interleukin-1
• Kinetics
• Liver (metabolism)
• Oligosaccharides (biosynthesis)
• Orosomucoid (biosynthesis)
• Proteins (pharmacology)
• Serum Albumin (biosynthesis)
• Sialyltransferases (analysis)