Experimental beryllium-induced lung disease. II. Analyses of bronchial lavage cells in strains 2 and 13 guinea pigs.

Abstract

In previous studies, we found that endotracheal administration of beryllium oxide (BeO) produced cell-mediated immunity and granulomatous lung disease in strain 2 but not strain 13 guinea pigs. This report describes phenotypic and functional studies of bronchial lavage cells from both guinea pig strains after BeO exposure. In BeO-treated strain 2 animals, the percentage of T (ER+)3 lymphocytes was significantly (p less than 0.05) elevated above normal by 2 weeks after BeO exposure, while T lymphocytes were not increased in strain 13. Giant cells containing crystalline material in fused, coalesced phagolysosomes were observed in treated animals of both strains. BeO-exposed strain 2 alveolar macrophages (AM) demonstrated enhanced killing of Listeria monocytogenes and enhanced chemiluminescence, while AM of strain 13 had responses below controls. Findings support the presence of BeO-induced cellular immunologic activity in strain 2 animals and, on the other hand, suggest BeO toxicity to lavage cells in strain 13.

• Authors: B P Barna, S D Deodhar, S Gautam, M Edinger, T Chiang, J T McMahon
• Journal: International archives of allergy and applied immunology (Int Arch Allergy Appl Immunol) Vol. 73 Issue 1 Pg. 49-55 ( 1984) ISSN: 0020-5915 [Print] Switzerland
• PMID: 6607221 (Publication Type: Journal Article, Research Support, U.S. Gov't, P.H.S.)

Chemical References

• beryllium oxide
• Muramidase
• Beryllium

Topics

• Animals
• Beryllium (pharmacology)
• Fibroblasts (pathology)
• Guinea Pigs
• Lung Diseases (chemically induced, immunology, pathology)
• Lymphocyte Activation (drug effects)
• Macrophages (enzymology, immunology, ultrastructure)
• Muramidase (metabolism)
• Neutrophils (immunology)
• Phagocytosis (drug effects)
• Pulmonary Alveoli (immunology, pathology)
• T-Lymphocytes (immunology)
• Therapeutic Irrigation