Human epidermal Langerhans cells induce cellular immune response to trichophytin in dermatophytosis.

Abstract

Human epidermal cell suspensions were prepared from patients sensitized to trichophytin. The cells were 75% to 95% viable, and contained 2% to 6% Langerhans cells as judged by immunofluorescence staining with an anti-DR antiserum. T lymphocytes from the same patients were co-cultured with trichophytin, with or without epidermal cells or macrophages. Proliferative T-cell response to trichophytin was obtained in cultures containing epidermal cells or macrophages. Pretreatment of the epidermal cells with anti-DR antiserum abolished the response, but normal rabbit serum did not, indicating that HLA-DR positive Langerhans cells are able to induce the cellular immune response to trichophytin. These data indicate that Langerhans cells play an important role in dermatophytosis.

• Authors: L R Braathen, T Kaaman
• Journal: The British journal of dermatology (Br J Dermatol) Vol. 109 Issue 3 Pg. 295-300 (Sep 1983) ISSN: 0007-0963 [Print] England
• PMID: 6604538 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• HLA-DR Antigens
• Histocompatibility Antigens Class II
• Trichophytin

Topics

• Dermatomycoses (immunology)
• HLA-DR Antigens
• Histocompatibility Antigens Class II (immunology)
• Humans
• Immunity, Cellular
• Langerhans Cells (immunology)
• Lymphocyte Activation
• Macrophages (immunology)
• T-Lymphocytes (immunology)
• Trichophytin (immunology)