Abstract |
The relative efficacy of the card test in the diagnosis of human brucellosis was evaluated by comparison with four other tests: the standard tube agglutination test, centrifugation agglutination test, 2-mercaptoethanol degradation technique, and 2-mercaptoethanol centrifugation agglutination test. A total of 1,701 serum specimens from persons with various degrees of potential exposure to brucella organisms or cross-reactive antigens were used in this study. In comparison with standard tube agglutination results, the card test had a sensitivity of 95.3% and a specificity of 84.1%. The card-test antigen was nonreactive with serum specimens from tularemia patients and cholera vaccinees. Our data indicated that this test measures both immunoglobulin G and immunoglobulin M agglutinins. The value of the card test in the presumptive serological diagnosis of clinical brucellosis in humans appears to be low; however, the card test may be of value in serological surveys to delineate high-risk populations.
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Authors | A O Russell, C M Patton, A F Kaufmann |
Journal | Journal of clinical microbiology
(J Clin Microbiol)
Vol. 7
Issue 5
Pg. 454-8
(May 1978)
ISSN: 0095-1137 [Print] United States |
PMID | 659575
(Publication Type: Comparative Study, Journal Article)
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Chemical References |
- Antibodies, Bacterial
- Immunoglobulin G
- Immunoglobulin M
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Topics |
- Agglutination Tests
- Antibodies, Bacterial
(analysis)
- Brucellosis
(diagnosis)
- Diagnosis, Differential
- Humans
- Immunoglobulin G
(analysis)
- Immunoglobulin M
(analysis)
- Serologic Tests
(methods)
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