Selective killing of leukemic cells by merocyanine 540-mediated photosensitization.

Abstract

In vitro incubation of leukemic bone marrow with the lipophilic fluorescent dye merocyanine 540 and simultaneous exposure to light reduced the concentration of L1210 leukemia cells by about 5 orders of magnitude but spared enough normal pluripotent hematopoietic stem cells to allow hematopoietic reconstitution of lethally irradiated syngeneic mice. This simple and rapid procedure may find an application in the purging of tumor cells from autologous bone marrow grafts.

• Authors: F Sieber, J L Spivak, A M Sutcliffe
• Journal: Proceedings of the National Academy of Sciences of the United States of America (Proc Natl Acad Sci U S A) Vol. 81 Issue 23 Pg. 7584-7 (Dec 1984) ISSN: 0027-8424 [Print] United States
• PMID: 6594700 (Publication Type: Journal Article, Research Support, U.S. Gov't, P.H.S.)

Chemical References

• Pyrimidinones
• Radiation-Sensitizing Agents
• merocyanine dye

Topics

• Animals
• Bone Marrow (drug effects, pathology, radiation effects)
• Cell Survival (drug effects)
• Colony-Forming Units Assay
• Hematopoietic Stem Cells (cytology, drug effects, radiation effects)
• Leukemia L1210 (pathology)
• Light
• Mice
• Pyrimidinones (toxicity)
• Radiation-Sensitizing Agents (toxicity)