HOMEPRODUCTSCOMPANYCONTACTFAQResearchDictionaryPharmaSign Up FREE or Login

On the dehydration of (R)-lactate in the fermentation of alanine to propionate by Clostridium propionicum.

Abstract
All the enzymes of the pathway of (S)-alanine fermentation to acetate and propionate were detected in cell-free extracts of Clostridium propionicum . Among these (S)-glutamate dehydrogenase (NAD), (R)-lactate dehydrogenase (NAD) and propionate CoA-transferase were purified to apparent homogeneity. Their structures were presumably alpha 6, alpha 2 and alpha 4, respectively. The latter enzyme was specific for short-chain monocarboxylic acids with a pronounced preference for (R)-lactate over the (S)-enantiomer. The key step of the pathway, the dehydration of (R)-lactate required acetyl phosphate and CoASH under anaerobic conditions. It was inhibited by hydroxylamine, arsenate, azide (1 mM each) or by 0.1 mM 2,4-dinitrophenol. Thus it closely resembled the dehydration of (R)-2-hydroxyglutarate in Acidaminococcus fermentans , although an activation was not necessary.
AuthorsG Schweiger, W Buckel
JournalFEBS letters (FEBS Lett) Vol. 171 Issue 1 Pg. 79-84 (Jun 04 1984) ISSN: 0014-5793 [Print] England
PMID6586495 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Lactates
  • Propionates
  • Lactic Acid
  • L-Lactate Dehydrogenase
  • Glutamate Dehydrogenase
  • Sulfurtransferases
  • Coenzyme A-Transferases
  • acyl-CoA transferase
  • propionic acid
  • Alanine
Topics
  • Alanine (metabolism)
  • Clostridium (metabolism)
  • Coenzyme A-Transferases
  • Fermentation
  • Glutamate Dehydrogenase (metabolism)
  • Kinetics
  • L-Lactate Dehydrogenase (metabolism)
  • Lactates (metabolism)
  • Lactic Acid
  • Propionates (metabolism)
  • Stereoisomerism
  • Sulfurtransferases (metabolism)

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research graph!


Choose Username:
Email:
Password:
Verify Password:
Enter Code Shown: