Thy 1.2+ leukemia cells eradicated from in vitro leukemia-bone marrow cell mixtures by antibody-toxin conjugates.

Abstract

A conjugate constituted by a monoclonal anti-Thy 1.2 antibody chemically coupled to the toxic subunit of ricin was synthesized. The conjugate was specifically cytotoxic for Thy 1.2+ (EL 4) but not for Thy 1.2- (BW5147) thymoma cells. In vitro treatment of bone marrow-leukemia cell mixtures (10:1 and 1:1 bone marrow to EL 4 cell ratios) with anti-Thy 1.2-ricin A chain totally eradicated EL 4 tumor cells, while the number of myeloid colony-forming units and pluripotent colony-forming units developed in vitro and in vivo was unaffected, which suggested that the treatment had no toxic side effects at least on the precursor cells examined.

• Authors: M Colombatti, A Colombatti, H E Blythman, C Bron
• Journal: Journal of the National Cancer Institute (J Natl Cancer Inst) Vol. 72 Issue 5 Pg. 1095-9 (May 1984) ISSN: 0027-8874 [Print] United States
• PMID: 6585586 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antibodies, Monoclonal
• Ricin

Topics

• Animals
• Antibodies, Monoclonal (immunology)
• Bone Marrow (immunology)
• Hematopoietic Stem Cells (drug effects)
• In Vitro Techniques
• Leukemia, Experimental (drug therapy, pathology)
• Mice
• Mice, Inbred BALB C
• Ricin (immunology, therapeutic use)