Human monoclonal antibody to a neuroectodermal tumor antigen (OFA-I-2).

Abstract

Human IgM kappa monoclonal antibody to human tumors of neuroectodermal origin was produced in the spent medium of an Epstein-Barr virus-transformed B-lymphoblastoid cell line, L72. Chemically, the antigen was identified as ganglioside GD2 [Gal NAc beta 1----4 (Neu Ac alpha 2----8 Neu Ac alpha 2---3) Gal beta 1----4 Glc----ceramide]. Twenty-seven mg of pure human IgM were obtained from 10 liters of L72 spent medium using salt and hypotonic precipitation, ultracentrifugation, and Sephacryl-S 300 superfine gel filtration. The monoclonal origin of the antibody was determined by agarose isoelectrofocusing. This human monoclonal antibody may be a particularly useful reagent for immunotherapy trials in cancer patients.

• Authors: M Katano, N Sidell, R F Irie
• Journal: Annals of the New York Academy of Sciences (Ann N Y Acad Sci) Vol. 417 Pg. 427-34 ( 1983) ISSN: 0077-8923 [Print] United States
• PMID: 6584083 (Publication Type: Journal Article, Research Support, U.S. Gov't, P.H.S.)

Chemical References

• Antibodies, Monoclonal
• Antigens, Neoplasm
• oncofetal antigens

Topics

• Antibodies, Monoclonal (immunology)
• Antigens, Neoplasm (immunology)
• Breast Neoplasms (immunology)
• Cell Line
• Colonic Neoplasms (immunology)
• Glioma (immunology)
• Humans
• Kidney Neoplasms (immunology)
• Leukemia, Lymphoid (immunology)
• Lung Neoplasms (immunology)
• Melanoma (immunology)
• Neuroblastoma (immunology)
• Sarcoma (immunology)