Nude mice as models for human leukemia studies.

Abstract

Human leukemic promyelocytes of the HL-60 line were grown as tumors in nude mice and studied. A single nonmetastatic granulocytic sarcoma developed after subcutaneous inoculation with HL-60 cells. Some exceeded 5 cm in size. Almost all mice developed tumors after initial priming with cyclophosphamide. Older sarcomas showed viable tumor islands in necrotic and fibrotic tissue. Some tumors appeared greenish. Histologic and electron-microscopic analysis demonstrated large, vaguely outlined cells in poorly vascularized sheets. The cells displayed high nucleocytoplasmic ratios, basophilic granular cytoplasms lacking Auer rods, and enzymes characteristic of cultured HL-60 promyelocytes. Some tumor cells also demonstrated monocyte/macrophage enzymes, such as butyrate esterase. Induced HL-60 tumors also corresponded morphologically to a human subcutaneous promyelocytic tumor specimen. Comparative cytologic studies of induced HL-60 tumors in nude mice and cultured HL-60 cells revealed virtual identity, suggesting the nude mouse tumor as a useful model for in vivo studies of human leukemic cells.

• Authors: G K Potter, R N Shen, J W Chiao
• Journal: The American journal of pathology (Am J Pathol) Vol. 114 Issue 3 Pg. 360-6 (Mar 1984) ISSN: 0002-9440 [Print] United States
• PMID: 6582788 (Publication Type: Journal Article, Research Support, U.S. Gov't, P.H.S.)

Chemical References

• Glucosephosphate Dehydrogenase

Topics

• Animals
• Cell Line
• Cell Transformation, Neoplastic (metabolism, pathology, ultrastructure)
• Disease Models, Animal
• Glucosephosphate Dehydrogenase (metabolism)
• Histocytochemistry
• Humans
• Leukemia, Myeloid, Acute (metabolism, pathology)
• Mice
• Mice, Nude
• Neoplasm Transplantation
• Sarcoma, Experimental (metabolism, pathology, ultrastructure)