Radioimmunoassay of free urinary 18-hydroxydeoxycorticosterone (18-OH-DOC) in patients with essential hypertension.

Abstract

Specific antiserum was raised in white New Zealand rabbits using 18-hydroxydeoxycorticosterone-3-oxime-BSA complex as antigen. The urinary free 18-OH-DOC was estimated after dichloromethane extraction and separation in one paper chromatographic system (propylene glycol/toluene). The mean 18-OH-DOC excretion value (+/- S.D.) in normal subjects was 0.861 +/- 0.527 microgram/24 h (n=23). ACTH produced a 25-fold increase in the excretion of free 18-OH-DOC. Dexamethasone suppressed the values to the lower range of sensitivity. 32% of patients of essential hypertension showed a moderate increase in the free urinary 18-OH-DOC values. The mean value (+/- S.D.) in the low renin hypertension group was 2.50 +/- 1.49 microgram/24 h (n=19), in the normal renin patient group 1.84 +/- 1.22 microgram/24 h (n=38). The difference between controls and the hypertensive groups was statistically significant. Among the different hypertensive groups significant differences could not be calculated.

• Authors: J Hornung, K H Gless, S Abdelhamid, W Vielhauer, P Vecsei
• Journal: Clinica chimica acta; international journal of clinical chemistry (Clin Chim Acta) Vol. 87 Issue 2 Pg. 181-7 (Jul 15 1978) ISSN: 0009-8981 [Print] Netherlands
• PMID: 657546 (Publication Type: Journal Article)

Chemical References

• 18-Hydroxydesoxycorticosterone
• Desoxycorticosterone
• Renin

Topics

• 18-Hydroxydesoxycorticosterone (urine)
• Desoxycorticosterone (analogs & derivatives)
• Evaluation Studies as Topic
• Humans
• Hypertension (enzymology, urine)
• Methods
• Radioimmunoassay
• Renin (blood)