Selective in vitro toxicity of purothionin conjugated to the monoclonal antibody 225.28S to a human high-molecular-weight melanoma-associated antigen.

Abstract

The toxic agent purothionin was conjugated to the monoclonal antibody 225.28S to a human high-molecular-weight melanoma-accociated antigen. The toxic conjugate displayed in vitro toxicity to cultured human Colo 38 melanoma cells as indicated by reduced uptake of 3H-thymidine following a 24-h incubation and loss of cell viability following a 7-day incubation. The effect is dose-dependent and is specific since addition of the toxic conjugate to a cultured Raji B lymphoid cells did not affect their 3H-thymidine uptake or their viability.

• Authors: K Imai, T Nakanishi, T Noguchi, A Yachi, S Ferrone
• Journal: Cancer immunology, immunotherapy : CII (Cancer Immunol Immunother) Vol. 15 Issue 3 Pg. 206-9 ( 1983) ISSN: 0340-7004 [Print] Germany
• PMID: 6555062 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)

Chemical References

• Antibodies, Monoclonal
• Antimicrobial Cationic Peptides
• Plant Proteins
• Staphylococcal Protein A
• DNA
• purothionin

Topics

• Antibodies, Monoclonal (administration & dosage)
• Antimicrobial Cationic Peptides
• Cell Survival
• Cells, Cultured
• DNA (biosynthesis)
• Humans
• Melanoma (immunology, therapy)
• Molecular Weight
• Plant Proteins (administration & dosage)
• Staphylococcal Protein A