Abstract |
Circulating tumor cells can be detected by means of flow cytometry in the blood of mice bearing i.m. Lewis lung carcinoma. This technique can be applied in the case of aneuploid tumors and does not require either concentration of nucleated cells or other processing of the blood samples. It offers the advantages of simplicity and speed and allows quantitative measurement of the number of circulating tumor cells. It can be applied to the study of the effects of drug treatment on the number of circulating tumor cells, for those drugs which do not cause alterations in the nuclear DNA content of normal diploid blood cells. The number of circulating tumor cells determined by flow cytometry is markedly reduced by treatment with ICRF-159, by dimethyltriazene DM-COOK, and also by its clinically used analog, DTIC.
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Authors | T Giraldi, G Sava, R Cherubino, G Bottiroli, G Mazzini |
Journal | Clinical & experimental metastasis
(Clin Exp Metastasis)
1984 Apr-Jun
Vol. 2
Issue 2
Pg. 151-9
ISSN: 0262-0898 [Print] Netherlands |
PMID | 6543695
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Antineoplastic Agents
- DNA, Neoplasm
- Piperazines
- Triazenes
- Razoxane
- 1-(4-carboxyphenyl)-3,3-dimethyltriazene
- Dacarbazine
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Topics |
- Animals
- Antineoplastic Agents
- Cell Line
- DNA, Neoplasm
(analysis)
- Dacarbazine
(therapeutic use)
- Female
- Flow Cytometry
- Lung Neoplasms
(ultrastructure)
- Mice
- Mice, Inbred C57BL
- Neoplasm Metastasis
(prevention & control)
- Neoplasms, Experimental
(drug therapy, ultrastructure)
- Neoplastic Cells, Circulating
- Piperazines
(therapeutic use)
- Razoxane
(therapeutic use)
- Triazenes
(therapeutic use)
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