[Spectrophotometric evaluation of hemolysis tests].

Abstract

A quantitative spectrophotometric method for measuring the hemolysis degree of PVC-biomaterials by potassium hexacyanoferrate (III) and potassium cyanide is presented. By means of this method the physiological valuation of biomaterials proved more exact as to their contents of hemolytically acting substances. In order to define the maximum permissible degree of hemolysis from biomaterials further studies will be necessary. UV- and IR spectrograms of the 70 degrees C and 121 degrees C extracts from biomaterials indicated the presence of substances not yet identified till now. These substances couldn't be ascribed any specific biological effects. An extraction at 121 degrees C is recommended in order to detect hemolytically acting substances.

• Authors: G Unger, C Ellinger, K Haberer
• Journal: Zeitschrift fur experimentelle Chirurgie, Transplantation, und kunstliche Organe : Organ der Sektion Experimentelle Chirurgie der Gesellschaft fur Chirurgie der DDR (Z Exp Chir Transplant Kunstliche Organe) Vol. 17 Issue 6 Pg. 337-43 ( 1984) ISSN: 0232-7295 [Print] Germany
• Vernacular Title: Zur spektrophotometrischen Auswertung des Hämolysetestes.
• PMID: 6516504 (Publication Type: Comparative Study, English Abstract, Journal Article)

Chemical References

• Biocompatible Materials
• Hemoglobins

Topics

• Biocompatible Materials (toxicity)
• Evaluation Studies as Topic
• Hemoglobins (analysis)
• Hemolysis (drug effects)
• Humans
• Spectrophotometry (methods)
• Temperature