Effects of 12-O-tetradecanoylphorbol-13-acetate on carcinogenesis in the heterotopically transplanted rat urinary bladder.

Abstract

12-O-Tetradecanoylphorbol-13-acetate (TPA), a potent promoter of mouse skin carcinogenesis, was tested for possible tumor-enhancing effects on urinary bladder carcinogenesis using the heterotopically transplanted bladder (HTB) model. Weekly administration of TPA at 1.0 microgram/week to N-methyl-N-nitrosourea-initiated HTBs did not increase tumor incidence, but instead, resulted in a significantly high incidence of nodulopapillary hyperplasia, an early neoplastic lesion, suggesting possible tumor enhancement by TPA. In addition, administration of a high dose of TPA with or without a carcinogen treatment led to the development of numerous finger-like epithelial projections on the luminal surface of the HTBs. Evidence indicates that epithelial projections are formed as a result of proliferation of intermediate cells. Whether these structures evolve into true neoplastic lesions is at present unknown.

• Authors: K Izumi, M Shibata, K Togei, A Akagi, H Otsuka, J B Jacobs, S Ozono, Y Miyata, R Oyasu
• Journal: Gan (Gan) Vol. 75 Issue 9 Pg. 756-62 (Sep 1984) ISSN: 0016-450X [Print] Japan
• PMID: 6500231 (Publication Type: Journal Article, Research Support, U.S. Gov't, P.H.S.)

Chemical References

• Phorbols
• Methylnitrosourea
• Tetradecanoylphorbol Acetate

Topics

• Animals
• Cell Division
• Male
• Methylnitrosourea
• Phorbols
• Rats
• Rats, Inbred F344
• Tetradecanoylphorbol Acetate
• Urinary Bladder (pathology, transplantation)
• Urinary Bladder Neoplasms (chemically induced, pathology, ultrastructure)