Induction of chromosome aberrations and sister-chromatid exchanges in CHO-K1 cells by o-phenylphenol.

Abstract

o-Phenylphenol (OPP), is used in Japan as a fungicide in food additives for citrus fruits. The induction of chromosome aberrations and sister-chromatid exchanges (SCEs) by OPP in cultured Chinese hamster ovary (CHO-K1) cells was studied. Cells were exposed to various concentrations of OPP ranging from 50 to 175 micrograms/ml for 3 h, and further incubated for 27 and 42 h. These incubation periods are almost equal to 2 and 3 cell cycles. SCEs and chromosome aberrations were induced by OPP at concentrations of 100, 125 and 150 micrograms/ml after the incubation for 27 h. For chromosome aberrations, chromatid breaks and exchanges there was a dose-dependent increase. Diplochromosomes due to endoreduplication were also caused by the same concentrations of OPP in a dose-dependent manner. After incubation for 42 h, chromosome aberrations were also increased by OPP at concentrations of 100 and 125 micrograms/ml, but the frequencies of SCEs were not significantly different from those of the control. These results suggest that OPP has a cytogenetic toxicity, and that the DNA damage resulting in SCEs induced by OPP is relatively short-lived and can be repaired during the longer incubation time.

• Authors: S Tayama-Nawai, S Yoshida, T Nakao, K Hiraga
• Journal: Mutation research (Mutat Res) Vol. 141 Issue 2 Pg. 95-9 (Oct 1984) ISSN: 0027-5107 [Print] Netherlands
• PMID: 6493272 (Publication Type: Journal Article)

Chemical References

• Biphenyl Compounds
• Methylnitronitrosoguanidine
• 2-phenylphenol

Topics

• Animals
• Biphenyl Compounds (toxicity)
• Cell Line
• Chromosome Aberrations
• Cricetinae
• Cricetulus
• Dose-Response Relationship, Drug
• Female
• Methylnitronitrosoguanidine (toxicity)
• Ovary
• Sister Chromatid Exchange (drug effects)
• Time Factors