Laboratory experience with radiometric detection of bacteremia with three culture media.

Abstract

In two long-term studies, the BACTEC radiometric system for detection of bacteremia was evaluated with three culture media each: (i) BACTEC media 6A (for aerobes) and 7B (for anaerobes) plus a thioglycolate medium and (ii) BACTEC media 6A, 7B, and 8A (hypertonic). In study 1, clinically significant isolates were identified in 1,873 (13.9%) of 13,432 blood cultures with all three media. The thioglycolate medium revealed 143 (1.1%) organisms not recovered from the 6A and 7B media. In study 2, isolates were identified in 1,135 (12.9%) of 8,759 cultures with all three media; 104 (1.2%) organisms were isolated only from the hypertonic medium. The increased yield of positive cultures in the three-medium system is likely due to the larger volume of blood cultured.

• Authors: K Wicher, D Koscinski
• Journal: Journal of clinical microbiology (J Clin Microbiol) Vol. 20 Issue 4 Pg. 668-71 (Oct 1984) ISSN: 0095-1137 [Print] United States
• PMID: 6490854 (Publication Type: Comparative Study, Journal Article)

Chemical References

• Carbon Radioisotopes
• Culture Media
• Hypertonic Solutions
• Thioglycolates

Topics

• Bacteria (isolation & purification)
• Carbon Radioisotopes
• Culture Media
• Humans
• Hypertonic Solutions
• Sepsis (microbiology)
• Thioglycolates