Abstract |
Chemical tumor promoters induce significant morphologic changes in several cultured cell models. In this article we describe a new effect of two potent, chemically different tumor promoters, 12-O-tetradecanoylphorbol-13-acetate (TPA) and dihydroteleocidin B (DHTB) on cultured human HeLa and melanoma cells. Using immunofluorescence microscopy, we observed that TPA and DHTB induced a dramatic increase in the size (greater than or equal to 3X normal diameter) of the centrosome, a microtubule-organizing center, within 24 h of incubation. In HeLa cells the effect was serum- and dose-dependent, was observed in 76-92% of cells within 72 h of incubation, and was associated with an increase in cytoplasm-nucleus ratio and proliferation of microtubules from the centrosome. The tumor promoters inhibited serum-induced DNA synthesis in both cell lines. Electron microscopy revealed the presence of clumps of microcentriole bodies or fragments adjacent to the intact centriole.
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Authors | R N Mascardo, P Sherline |
Journal | The Journal of clinical investigation
(J Clin Invest)
Vol. 74
Issue 4
Pg. 1186-92
(Oct 1984)
ISSN: 0021-9738 [Print] United States |
PMID | 6480823
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)
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Chemical References |
- Growth Inhibitors
- Lyngbya Toxins
- Phorbols
- DNA
- dihydroteleocidin B
- Tetradecanoylphorbol Acetate
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Topics |
- DNA
(biosynthesis)
- Growth Inhibitors
(pharmacology)
- HeLa Cells
(drug effects, pathology)
- Humans
- Hyperplasia
(chemically induced)
- Kinetics
- Lyngbya Toxins
(pharmacology)
- Melanoma
(metabolism, pathology)
- Microtubules
(drug effects, pathology)
- Mitotic Index
(drug effects)
- Organoids
(drug effects, pathology)
- Phorbols
(pharmacology)
- Tetradecanoylphorbol Acetate
(pharmacology)
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