Abstract |
The effect of the cationic permeant fluorescent dye, rhodamine 123 (R123), on the in vivo growth of Plasmodium yoelii was examined. Plasmodium yoelii-infected mouse erythrocytes were incubated in vitro with R123 and injected intravenously into mice. Examination of daily parasitemias showed that R123 delayed parasite growth whereas rhodamine 110, a neutral compound, and fluorescein, a negatively charged fluorescent dye, did not. Infected erythrocytes treated with R123 were not cleared from the circulation even 7 h after injection. Quantitation of cell-associated R123 by spectrophotometry revealed that infected cells with increased levels of R123 considerably prolonged the 2% prepatent period, the time required for the parasite to develop a 2% parasitemia. Degenerating parasites within and outside the host erythrocytes were observed on day 1 of infection in the mice. Thus it follows that R123, which accumulated in infected erythrocytes, inhibits the growth of P. yoelii; moreover, when R123-labeled infected erythrocytes were treated with 1-10 microM carbonylcyanide m-chlorophenylhydrazone ( CCCP), a proton ionophore, to release R123 from the cells, the inhibitory effect on the growth rate of P. yoelii was partially reversed.
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Authors | K Tanabe |
Journal | The Journal of protozoology
(J Protozool)
Vol. 31
Issue 2
Pg. 310-3
(May 1984)
ISSN: 0022-3921 [Print] United States |
PMID | 6470989
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Rhodamines
- Xanthenes
- Rhodamine 123
- Carbonyl Cyanide m-Chlorophenyl Hydrazone
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Topics |
- Animals
- Carbonyl Cyanide m-Chlorophenyl Hydrazone
(pharmacology)
- Erythrocytes
(metabolism, parasitology)
- Female
- Malaria
(parasitology)
- Mice
- Mice, Inbred ICR
- Plasmodium
(drug effects, growth & development)
- Rhodamine 123
- Rhodamines
(blood, pharmacology)
- Xanthenes
(pharmacology)
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