Xeroderma pigmentosum fibroblasts of the D group lack an apurinic DNA endonuclease species with a low apparent Km.

Abstract

Apurinic DNA endonuclease activity from cultured human fibroblasts was resolved into two species by phosphocellulose chromatography. The species had sedimentation coefficients of 3.3 S and 2.8 S and apparent Km's for apurinic sites of 5 and 44 nM, respectively. The low Km species was absent from extracts of cell lines XP5BE, XP6BE and XP7BE of xeroderma pigmentosum complementation group D.

• Authors: U Kuhnlein, B Lee, E E Penhoet, S Linn
• Journal: Nucleic acids research (Nucleic Acids Res) Vol. 5 Issue 3 Pg. 951-60 (Mar 1978) ISSN: 0305-1048 [Print] England
• PMID: 643622 (Publication Type: Comparative Study, Journal Article, Research Support, U.S. Gov't, Non-P.H.S.)

Chemical References

• Apurinic Acid
• Deoxyribonucleases
• Endonucleases

Topics

• Apurinic Acid
• Cell Line
• Deoxyribonucleases (deficiency, isolation & purification)
• Endonucleases (deficiency, isolation & purification)
• Humans
• Kinetics
• Substrate Specificity
• Xeroderma Pigmentosum (enzymology, genetics)