Abstract |
The metabolism of 14C-PMM and its irreversible interaction with DNA and proteins were studied in M5076/73A reticular cell sarcoma, a murine solid tumor previously shown to be sensitive to the drug. Metabolism and irreversible binding were determined 0.25, 1, 8 and 104 hours after a single i.p. injection of radiolabelled PMM, tumor and liver macromolecular binding were compared with two differently 14C-labelled PMM, i.e. ring- and methyl-PMM. Ring-PMM derived macromolecular binding appeared to have more relevance in vivo and had a similar time profile in both liver and tumor. Ring-PMM derived DNA binding was then related to metabolic steps between PMM and 2,2,4,6 TMM and 2,2,4,6 TMM itself and 2,4,6 TriMM.
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Authors | E Garattini, M Broggini, P Coccia, T Colombo, C Rossi, M D'Incalci |
Journal | Biochemical pharmacology
(Biochem Pharmacol)
Vol. 33
Issue 17
Pg. 2715-22
(Sep 01 1984)
ISSN: 0006-2952 [Print] England |
PMID | 6431992
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Antineoplastic Agents
- DNA, Neoplasm
- Neoplasm Proteins
- Triazines
- Altretamine
- pentamethylmelamine
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Topics |
- Altretamine
(analogs & derivatives, metabolism)
- Animals
- Antineoplastic Agents
(metabolism)
- DNA, Neoplasm
(metabolism)
- Female
- Liver
(metabolism)
- Lymphoma, Large B-Cell, Diffuse
(metabolism)
- Mice
- Mice, Inbred C57BL
- Neoplasm Proteins
(metabolism)
- Ovarian Neoplasms
(metabolism)
- Protein Binding
- Triazines
(metabolism)
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