Localization of adult T-cell leukemia-associated antigens by the immunocolloidal gold method.

Abstract

Adult T-cell leukemia-associated antigen (ATLA) and adult T-cell leukemia virus (ATLV) antigens were localized in the MT-2 cell system by the immunocolloidal gold method using 71 human sera having various anti-ATLA titers and rabbit anti-ATLV antisera. In the thin-section method with anti-ATLA-positive human sera and rabbit antisera, protein A-gold particles were preferentially observed on and around sectioned adult T-cell leukemia (ATL) virions located in pericellular aggregates and within the cytoplasmic vacuoles but nowhere else in a significant number. The number of gold particles per ATL virion was statistically well correlated with the anti-ATLA titers of human sera applied (p less than 0.005). The preembedding method showed that pericellular ATL virions were specifically tagged with protein A-gold, but the antigens in question were not expressed on the plasma membrane of MT-2 cells. The absence of ATLA and ATLV antigens on the plasma membrane constitutes a unique pathobiological feature of ATL and ATLV as compared with other retrovirus systems.

• Authors: H Tanaka, S Haga, K Takatsuki, K Yamaguchi
• Journal: Cancer research (Cancer Res) Vol. 44 Issue 8 Pg. 3493-504 (Aug 1984) ISSN: 0008-5472 [Print] United States
• PMID: 6430551 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antigens, Neoplasm
• Colloids
• Immune Sera
• Indicators and Reagents
• Membrane Glycoproteins
• thymus-leukemia antigens
• Gold

Topics

• Adult
• Antigens, Neoplasm (analysis)
• Cell Line
• Cell Membrane (immunology)
• Colloids
• Gold
• Humans
• Immune Sera
• Immunodiffusion
• Indicators and Reagents
• Leukemia (immunology)
• Membrane Glycoproteins
• T-Lymphocytes (immunology)