Increased sensitivity of IL2-dependent cultured T cells and enhancement of in vitro IL2 production by human lymphocytes treated with Bestatin.

Abstract

Bestatin is an inhibitor of leucine aminopeptidase and aminopeptidase B which potentiates various immune functions, such as delayed hypersensitivity and antibody formation, and inhibits tumor cell growth in animal models. Our study focuses on the effect of Bestatin on interleukin 2 (IL2) sensitivity of IL2-dependent cultured T cells (CTC) and on IL2 production by human peripheral blood mononuclear cells (PBM). Bestatin (0.01 - 10 micrograms/ml) increases CTC 3H-TdR incorporation in the presence of suboptimal (low) concentrations of exogenous IL2. Bestatin alone has no mitogenic effect on CTC. This phenomenon is due to increased CTC IL2-receptors after treatment with Bestatin. Bestatin (1 - 10 micrograms/ml) also increases (1.4 - 21 fold) IL2 production by PHA-stimulated PBM. Bestatin therefore increases both IL2 sensitivity and IL2 production. This may explain the various immunopotentiating effects of Bestatin previously described.

• Authors: T Noma, B Klein, D Cupissol, J Yata, B Serrou
• Journal: International journal of immunopharmacology (Int J Immunopharmacol) Vol. 6 Issue 2 Pg. 87-92 ( 1984) ISSN: 0192-0561 [Print] England
• PMID: 6429060 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Adjuvants, Immunologic
• Interleukin-2
• Receptors, Immunologic
• Receptors, Interleukin-2
• Leucine
• ubenimex

Topics

• Adjuvants, Immunologic (pharmacology)
• Humans
• Immune Adherence Reaction
• In Vitro Techniques
• Interleukin-2 (biosynthesis, physiology)
• Leucine (analogs & derivatives, pharmacology)
• Lymphocyte Activation (drug effects)
• Receptors, Immunologic (drug effects)
• Receptors, Interleukin-2
• T-Lymphocytes (immunology)