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Phosphoproteins altered by antiproliferative doses of human interferon- beta in a human bladder carcinoma cell line.

Abstract
Phosphoproteins of control and IFN-beta treated human bladder carcinoma cells (RT4) were labelled in vitro with [32P]-ATP and analyzed by polyacrylamide gel electrophoresis. Cells treated with antiproliferative doses of IFN had reduced levels of phosphorylated 60 Kd and 40 Kd proteins. IFN also induced within 24 hours the modification of a low molecular weight phosphoprotein doublet in the 22-24,000 molecular weight range. The ability to phosphorylate high molecular weight proteins by the in vitro procedures was generally depressed by IFN treatment. There was a dramatic shift in the phosphorylation of alkali stable phosphoamino acids associated with proteins in the 43-50,000 molecular weight range in IFN-treated cells. Preliminary studies indicate that at least some of the IFN-induced modifications of cellular phosphoproteins may result from transcriptional control of specific oncogenes.
AuthorsG Soslau, A R Bogucki, D Gillespie, H R Hubbell
JournalBiochemical and biophysical research communications (Biochem Biophys Res Commun) Vol. 119 Issue 3 Pg. 941-8 (Mar 30 1984) ISSN: 0006-291X [Print] United States
PMID6424675 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)
Chemical References
  • Phosphoproteins
  • Phosphorus Radioisotopes
  • Interferon-gamma
  • Adenosine Triphosphate
Topics
  • Adenosine Triphosphate (metabolism)
  • Cell Cycle (drug effects)
  • Cell Line
  • Humans
  • Interferon-gamma (toxicity)
  • Kinetics
  • Molecular Weight
  • Phosphoproteins (isolation & purification, metabolism)
  • Phosphorus Radioisotopes
  • Urinary Bladder Neoplasms (metabolism)

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