Regulation of TCGF production in T cells. II. Early membrane events after anti-Thy-1 binding by the TCGF-producing T lymphoma EL-4 G-12.

Abstract

The T cell lymphoma line EL-4 G-12 produces large quantities of TCGF (IL 2) shortly after exposure to anti-Thy-1 and cross-linking reagents. Early surface events that initiate nonconstitutive synthesis and secretion have been examined by fluorescent microscopy, EM analysis, flow cytometry of anti-Thy-1, and measurements of membrane polarization. The data show that anti-Thy-1 binding leads to immediate and rapid endocytosis and shedding of the antibody molecules, but with no change in membrane potential. Cross-linking of the anti-Thy-1 results in stabilization and accumulation of the antibody on the surface, and rapid hyperpolarization. The data suggest that cross-linking of ligand antibody interactions at the surface is a regulatory event involved in initiation of the signal for TCGF synthesis and secretion.

• Authors: M A Norcross, R T Smith, S Shimizu
• Journal: Journal of immunology (Baltimore, Md. : 1950) (J Immunol) Vol. 132 Issue 2 Pg. 833-8 (Feb 1984) ISSN: 0022-1767 [Print] United States
• PMID: 6418811 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)

Chemical References

• Antibodies, Anti-Idiotypic
• Antilymphocyte Serum
• Cross-Linking Reagents
• Fluoresceins
• Interleukin-2
• Thiocyanates
• Fluorescein-5-isothiocyanate

Topics

• Animals
• Antibodies, Anti-Idiotypic (physiology)
• Antilymphocyte Serum (immunology)
• Binding Sites, Antibody
• Cell Line
• Cell Membrane (physiology)
• Cross-Linking Reagents (pharmacology)
• Endocytosis
• Fluorescein-5-isothiocyanate
• Fluoresceins
• Fluorescent Antibody Technique
• Goats
• Interleukin-2 (biosynthesis)
• Lymphoma (immunology)
• Membrane Potentials
• Mice
• Rabbits
• T-Lymphocytes (immunology)
• Thiocyanates