Conversion of 5'-deoxy-5'-methylthioadenosine and 5'-deoxy-5'-methylthioinosine to methionine in cultured human leukemic cells.

Abstract

5'-Deoxy-5'-methylthioadenosine and 5'-deoxy-5'-methylthioinosine, which are metabolized to the methionine precursor, 5-methylthioribose-1-phosphate, by 5'-deoxy-5'-methylthioadenosine phosphorylase and purine nucleoside phosphorylase, respectively, can serve as sources of methionine for cultured HL-60 promyelocytic leukemia cells. CCRF-CEM T-cell leukemia cells, which lack 5'-deoxy-5'-methylthioadenosine phosphorylase, convert 5'-deoxy-5'-methylthioinosine (but not 5'-deoxy-5'-methylthioadenosine) to methionine; this conversion is blocked by purine nucleoside phosphorylase inhibitors. Therefore, the pathway for the conversion of 5-methylthioribose-1-phosphate to methionine is present in both human leukemic lines.

• Authors: T M Savarese, L Y Ghoda, D L Dexter, R E Parks Jr
• Journal: Cancer research (Cancer Res) Vol. 43 Issue 10 Pg. 4699-702 (Oct 1983) ISSN: 0008-5472 [Print] United States
• PMID: 6411330 (Publication Type: Journal Article, Research Support, U.S. Gov't, P.H.S.)

Chemical References

• Deoxyadenosines
• Thionucleosides
• 5'-methylthioinosine
• Methylthioinosine
• Inosine
• 5'-methylthioadenosine
• Methionine
• Purine-Nucleoside Phosphorylase
• Adenosine

Topics

• Adenosine (analogs & derivatives, metabolism)
• Cells, Cultured
• Deoxyadenosines
• Humans
• Inosine (analogs & derivatives)
• Leukemia (metabolism)
• Leukemia, Myeloid, Acute (metabolism)
• Methionine (metabolism)
• Methylthioinosine (metabolism)
• Purine-Nucleoside Phosphorylase (antagonists & inhibitors)
• T-Lymphocytes
• Thionucleosides (metabolism)