Amino acid sequence of the Fv region of a human monoclonal IgM (protein WEA) with antibody activity against 3,4-pyruvylated galactose in Klebsiella polysaccharides K30 and K33.

Abstract

We have determined the amino acid sequence of the Fv [variable heavy (VH) and variable light (VL)] region of a human monoclonal IgM-kappa with antibody activity against 3,4-pyruvylated galactose, isolated from the plasma of patient WEA with Waldenström macroglobulinemia. The VH region has 114 residues, belongs to subgroup III, and has a very short third complementarity-determining region (CDR3), probably due to a small D segment/or an unusual D-J rearrangement (D, diversity; J, joining). The VL region has 108 residues and belongs to subgroup V kappa I. Compared to other members of the human VHIII and V kappa I families, WEA Fv does not appear to have significant differences within the framework residues but has unique CDRs that might be responsible for the particular antibody activity. Another IgM-kappa (GAL), which has an as-yet-undetermined antibody activity, shares a striking homology in V kappa with WEA, including an identical CDR1.

• Authors: F Goñi, B Frangione
• Journal: Proceedings of the National Academy of Sciences of the United States of America (Proc Natl Acad Sci U S A) Vol. 80 Issue 15 Pg. 4837-41 (Aug 1983) ISSN: 0027-8424 [Print] United States
• PMID: 6410398 (Publication Type: Journal Article, Research Support, U.S. Gov't, P.H.S.)

Chemical References

• Antibodies, Monoclonal
• Antigen-Antibody Complex
• Immunoglobulin Fab Fragments
• Immunoglobulin Fragments
• Immunoglobulin Variable Region
• Polysaccharides, Bacterial
• 3,4-pyruvylated galactose
• Cyanogen Bromide
• Galactose

Topics

• Amino Acid Sequence
• Antibodies, Monoclonal (immunology)
• Antigen-Antibody Complex
• Binding Sites, Antibody (immunology)
• Cyanogen Bromide
• Galactose (analogs & derivatives, immunology)
• Humans
• Immunoglobulin Fab Fragments (immunology)
• Immunoglobulin Fragments (analysis)
• Immunoglobulin Variable Region (immunology)
• Klebsiella (immunology)
• Polysaccharides, Bacterial (immunology)
• Waldenstrom Macroglobulinemia (immunology)