Apolipoprotein C-II deficiency associated with nonfunctional mutant forms of apolipoprotein C-II.

Abstract

Two previously unidentified apolipoproteins (apo) designated apo C-II-X and C-II-Y have been found in plasma of homozygotes and obligate heterozygotes of a family with apo C-II deficiency. Because the plasma of homozygotes do not activate lipoprotein lipase, apo C-II-X and C-II-Y are apparently nonfunctional. These apolipoproteins have isoelectric focusing points of 5.15 and 5.54, respectively, compared with 4.88 and 4.74 for the known isomorphs, C-II-1 and C-II-2, respectively. They have approximately similar molecular weights to apo C-II-1 and C-II-2 on two-dimensional sodium dodecyl sulphate-glycerol-polyacrylamide slab gel electrophoresis. They do not form insoluble antigen-antibody complexes with antibodies to apo C-II in single antibody immunodiffusion or electroimmunoassay systems. However, using a double-antibody technique in which immunoblotting is coupled with polyacrylamide isoelectric focusing slab gel electrophoresis, apo C-II-1, C-II-2, C-II-X, and C-II-Y have similar reactivity with antibodies to apo C-II. In this family the presence of apo C-II-X and C-II-Y discriminates obligate heterozygotes from normal subjects.

• Authors: G F Maguire, J A Little, G Kakis, W C Breckenridge
• Journal: Canadian journal of biochemistry and cell biology = Revue canadienne de biochimie et biologie cellulaire (Can J Biochem Cell Biol) Vol. 62 Issue 9 Pg. 847-52 (Sep 1984) ISSN: 0714-7511 [Print] Canada
• PMID: 6388756 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Apolipoprotein C-II
• Apolipoproteins C
• Lipoproteins, VLDL
• Lipoprotein Lipase

Topics

• Apolipoprotein C-II
• Apolipoproteins C (deficiency, genetics)
• Enzyme Activation
• Female
• Heterozygote
• Homozygote
• Humans
• Immunosorbent Techniques
• Isoelectric Focusing
• Lipoprotein Lipase (blood)
• Lipoproteins, VLDL (blood)
• Male
• Mutation