Abstract |
A single intravenous injection into rats of 0.4 mg of the muralytic enzyme mutanolysin, given as long as 3 d after an arthropathic dose of peptidoglycan- polysaccharide polymers derived from group A streptococci (PG-APS), resulted in a complete resolution of acute arthritis and the prevention of chronic joint disease. When administration of mutanolysin was delayed until 14 d after the injection of PG-APS, a great reduction in the severity of chronic inflammation was still observed. Quantitation of the amount of PG-APS present in the limbs, spleen, and liver by a solid phase enzyme-linked immunoassay indicated that the tissues of mutanolysin-treated rats contained as much PG-APS as tissues of PBS-treated control rats. In addition, rats treated with mutanolysin immediately after receiving an intraperitoneal injection of PG-APS developed a transient limb edema similar to that seen in rats after the injection of PG-APS digested to a small fragment size in vitro with mutanolysin. We hypothesize that mutanolysin acts in vivo by degrading PG-APS to small fragments that persist but are no longer arthropathic.
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Authors | M J Janusz, C Chetty, R A Eisenberg, W J Cromartie, J H Schwab |
Journal | The Journal of experimental medicine
(J Exp Med)
Vol. 160
Issue 5
Pg. 1360-74
(Nov 01 1984)
ISSN: 0022-1007 [Print] United States |
PMID | 6387033
(Publication Type: Journal Article, Research Support, U.S. Gov't, P.H.S.)
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Chemical References |
- Peptidoglycan
- Acetylmuramyl-Alanyl-Isoglutamine
- peptidoglycan pentapeptide-mDap3
- Muramidase
- Endopeptidases
- mutanolysin
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Topics |
- Acetylmuramyl-Alanyl-Isoglutamine
(analogs & derivatives)
- Animals
- Arthritis
(drug therapy, etiology, pathology)
- Cell Wall
(enzymology)
- Chronic Disease
- Endopeptidases
(administration & dosage, therapeutic use)
- Female
- Injections, Intravenous
- Liver
(pathology)
- Lymph Nodes
(pathology)
- Muramidase
(metabolism)
- Peptidoglycan
- Rats
- Rats, Inbred Lew
- Spleen
(pathology)
- Streptococcus pyogenes
(enzymology)
- Tarsal Joints
(pathology)
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